Table 1. Strains and plasmids used in this study.
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| Po1f | MATa, leu2-270, ura3-302, xpr2-322, axp-2 | ATCC [39] |
| Δku70 | Po1f ku70Δ | This study |
| Δpex10 URA3 | Po1f pex10Δ :: ura3 | This study |
| Δku70 Δpex10 URA3 | Po1f ku70Δ pex10Δ :: ura3 | This study |
| crtI URA3 | Po1f ku70Δ pox1Δ :: crtI ura3 | This study |
| crtI | Po1f ku70Δ pox1Δ :: crtI | This study |
| crtI/YB URA3 | Po1f ku70Δ pox1Δ :: crI pox2Δ :: crtYB ura3 | This study |
| crtI/YB | Po1f ku70Δ pox1Δ :: crI pox2Δ :: crtYB | This study |
| crtI/YB/E URA3 | Po1f ku70Δ pox1Δ :: crtI pox2Δ :: crtYB pox3Δ :: crtE ura3 | This study |
| crtI/YB/E | Po1f ku70Δ pox1Δ :: crtI pox2Δ :: crtYB pox3Δ :: crtE | This study |
| Plasmids | ||
| pUC57-URA3 | Plasmid containing 3HA-URA3-3HA deletion cassette | [40] |
| pYLEX1 | JMP62-LEU | Yeasterna |
| pUC-ylURA3 | Plasmid containing 3HA-ylURA3-3HA deletion cassette | This study |
| pUC-ylURA3-P4UAS1B | Plasmid containing P4UAS1B, TXPR2, 3HA-ylURA3-3HA integration cassette | This study |
| pUC-ylURA3-crtI | crtI ORF cloned between the PmlI and KpnI site of pUC-ylURA3-P4UAS1B | This study |
| pUC-ylURA3-crtYB | crtYB ORF cloned between the PmlI and KpnI site of pUC-ylURA3-P4UAS1B | This study |
| pUC-ylURA3-crtE | crtE ORF cloned between the PmlI and KpnI site of pUC-ylURA3-P4UAS1B | This study |
aThe pYLEX1 plasmid was purchased from Yeastern Biotech Company (Taipei, Taiwan).