Table 2. Efficiency of gene deletion.

Cells treated or untreated with HU were transformed with a pex10-deletion cassette with 50 bp of homology arm to the pex10 gene. The pex10 deletion rates (%) are shown and the number of total transformants screened is included in parentheses. WT indicates the wild-type Y. lipolytica Po1f strain. The experiments were performed in duplicate.

Strains	Target gene	Targeting homology length	Transformation condition
			Untreated cells % gene targeting (total transformants screened)	HU-treated cells % gene targeting (total transformants screened)
WT	pex10	50 bp	0% (20)	90% (20)
Δku70			0% (20)	60% (35)