Table 2. Microscopic parameters and effective macroscopic rate constants for reversible/irreversible bimolecular reactions of the form A + B ↔ C.
| Usage | Reaction | Membrane on/off rates | Mean fitted | ||||
|---|---|---|---|---|---|---|---|
| kAon (1/s) |
kAoff (1/s) |
kBon (1/s) |
kBoff (1/s) |
kf | kr (1/s) | ||
| 2D | Cdc42Dm + BemGEFm → Cdc42T | 36 | 13 | 10 | 40 | 0.040 μm2s-1 | - |
| Cdc42Dm + BemGEF42 → Cdc42T | 36 | 13 | - | - | 0.184 μm2s-1 | - | |
| BemGEFm + Cdc42T ↔ BemGEF42 | 10 | 40 | - | - | 0.054 μm2s-1 | 31.4 | |
| q3D | Cdc42Dm + BemGEFm → Cdc42T | 36 | 0.65 | 10 | 10 | 0.16 μM-1s-1 | - |
| Cdc42Dm + BemGEF42 → Cdc42T | 36 | 0.65 | - | - | 0.16 μM-1s-1 | - | |
| BemGEFm + Cdc42T ↔ BemGEF42 | 10 | 10 | - | - | 0.79 μM-1s-1 | 0.37 | |
The rates constants kAon and kBon are the rates at which the reactants, A and B, associate with the membrane, respectively, and the rate constants kAoff and kBoff are the corresponding off rates. Mean fits were computed over simulations using five separate initial conditions; see Table A in S1 Text for more details, and Table 3 for pre-fitting target parameters. These values refer to parameters used in Fig 3.