Table 1. Under-expressed sporulation genes in R20291::sinRR’.
| Locus Tag | Gene | Protein Name | Fold-Change: R20291/sinRR’ mutant |
log2 ratio | Known/predicted sigma factor needed for expression | Adjusted p value |
|---|---|---|---|---|---|---|
| CDR20291_0104 | cwlD | Germination N-acetylmuramoyl-L-alanine amidase, Autolysin | 67.7 | 6.1 | SigE | 1.01E-09 |
| CDR20291_0125 | spoIIID | Stage III sporulation protein D | 68.0 | 6.1 | SigE | 6.24E-05 |
| CDR20291_0128 | putative sporulation protein yyac, DUF1256 family | 50.2 | 5.6 | SigE | 0.005 |
|
| CDR20291_0213 | hypothetical protein | 3041.9 | 11.6 | SigE | 6.05E-14 |
|
| CDR20291_0316 | spore coat assembly asparagine rich protein | 81.8 | 6.4 | SigE | 0.00057 |
|
| CDR20291_0713 | Putative sporulation protein YunB | 63.0 | 6.0 | SigE | 0.00666 |
|
| CDR20291_1005 | Putative membrane protein, BDBH YlbJ involved in spore cortex formation | 5.7 | 2.5 | SigE | 5.93E-06 |
|
| CDR20291_1030 | spoIIIAA | Stage III sporulation protein AA | 18464.8 | 14.2 | SigE | 0.00005 |
| CDR20291_1031 | spoIIIAB | Stage III sporulation protein AB | 90.8 | 6.5 | SigE | 8.33E-06 |
| CDR20291_1033 | spoIIIAD | Stage III sporulation protein AD | 3571.7 | 11.8 | SigE | 1.32E-08 |
| CDR20291_1034 | spoIIIAE | Stage III sporulation protein AE | 13.9 | 3.8 | SigE | 1.48E-09 |
| CDR20291_1035 | spoiIIIAF | Stage III sporulation protein AF | 119.5 | 6.9 | SigE | 0.00825 |
| CDR20291_1036 | spoIIIAG | Stage III sporulation protein AG | 794.0 | 9.6 | SigE | 2.08E-09 |
| CDR20291_1051 | spoIVB | Stage IV sporualtion protein AB | 6.2 | 2.6 | SigE, SigG | 6.61E-06 |
| CDR20291_1073 | Putative phage protein, skin element | 98.9 | 6.6 | SigE | 0.00821 |
|
| CDR20291_1282 | cotE | Spore coat protein CotE peroxiredoxin/chitinase | 18.7 | 4.2 | SigE | 0.00029 |
| CDR20291_1360 | cotB | Spore outer coat layer protein CotB | 332.0 | 8.4 | SigE | 1.03E-07 |
| CDR20291_2146 | cspC | Subtilisin-like serine germination related protease- CspC | 16.3 | 4.0 | SigE | 0.00921 |
| CDR20291_2147 | cspBA | Subtilisin like serine germination related protease, CspB | 19.5 | 4.3 | SigE | 7.88E-07 |
| CDR20291_2289 | cotJA | Putative spore coat protein | 103 | 7.0 | SigE | 0.00074 |
| CDR20291_2291 | cotD | Spore coat protein CotD manganese catalase | 139.6 | 7.1 | SigE | 0.056 |
| CDR20291_2334 | spoIV | Stage IV sporulation protein | 4.1 | 2.0 | SigE | 9.981E-06 |
| CDR20291_2335 | putative sporulation protein yyac | 1593.4 | 10.6 | SigE | 0.00097 | |
| CDR20291_2513 | spoIVA | Stage IV sporulation protein AA | 309.1 | 8.3 | SigE | 2.35E-06 |
| CDR20291_2573 | spoIIE | Stage II sporulation protein E | 36.13938 | 5.17 | SigE | 7.04E-05 |
| CDR20291_3331 | Putative spore protein | 3.1 | 1.6 | SigE | 6.75E-09 |
|
| CDR20291_3376 | spmB | Spore maturation protein B | 41.4 | 5.4 | SigE | 0.00081 |
| CDR20291_3377 | spmA | Spore maturation protein A | 21.5 | 4.4 | SigE | 2.80E-12 |
| CDR20291_3404 | sipL | SpoIVA interacting protein | 18.3 | 4.2 | SigE | 8.71E-05 |
| CDR20291_0124 | spoIIQ | Stage II sporulation protein Q | 56.9 | 5.8 | SigF | 0.05 |
| CDR20291_0213 | hypothetical protein | 3041.9 | 11.6 | SigE, SigF | 6.05E-14 |
|
| CDR20291_0316 | spore coat assembly asparagine-rich protein | 81.8 | 6.4 | SigE, SigF | 0.0005 |
|
| CDR20291_2362 | spoIIP | Stage II sporulation protein P | 15.1 | 3.9 | SigF | 2.67E-11 |
| CDR20291_2363 | gpr | Spore endopeptidase | 28.8 | 4.8 | SigF | 1.83E-07 |
| CDR20291_2576 | sspA | small acid-soluble spore protein A | 196.8 | 7.6 | SigG, SigF | 5.77E-07 |
| CDR20291_3080 | small acid-soluble spore protein | 9.0 | 3.2 | SigG, SigF | 6.98E-06 |
|
| CDR20291_3107 | sspB | small acid-soluble spore protein B | 305.5 | 8.3 | SigG, SigE, SigF | 5.95E-08 |
| CDR20291_3400 | sleB | Putative spore cortex-lytic enzyme | 14.0 | 3.8 | SigF | 5.79E-09 |
| CDR20291_2530 | sigG | RNA polymease sigma-G factor | 44.0 | 5.5 | SigG | 2.10E-11 |
| CDR20291_0702 | spoVAC | Stage V sporulation protein VAC | 86.6 | 6.4 | SigG | 0.000105 |
| CDR20291_0703 | spoVAD | Stage V sporualtion protein VAD | 84.5 | 6.4 | SigG | 0.000161 |
| CDR20291_3080 | small acid-soluble spore protein | 9.0 | 3.2 | SigG | 6.98E-06 |
|
| CDR20291_3336 | spoVT | Stage V sporulation protein T | 309.1 | 8.3 | SigG | 9.59E-10 |
| CDR20291_0476 | sleC | SleC- spore peptidoglycan hydrolase/ germinant receptor complex | 642.4 | 9.3 | SigE, SigK | 0.00506 |
| CDR20291_0926 | cdeC | Cysteine rich exosporium protein | 101.6 | 6.7 | SigE, SigF, SigK | 1.05E-06 |
| CDR20291_1282 | cotE | Spore coat protein CotE peroxiredoxin/chitinase | 18.7 | 4.2 | SigE, SigK | 0.00029 |
| CDR20291_2289 | cotJA | Putative spore coat protein | 103 | 7.0 | SigE, SigK | 0.00074 |
| CDR20291_2291 | cotD | Spore coat protein CotD manganese catalase | 139.6 | 7.1 | SigE, SigK | 0.056 |
| CDR20291_3090 | bclA2 | exosprium glycoprotein | 8.4 | 3.1 | SigE, SigF, SigK | 0.00288 |
| CDR20291_3193 | bclA3 | Putative exosporium glycoprotein | 13.1 | 3.7 | SigE, SigF, SigK | 0.00543 |