Fig 6. cis4Δ and zrg17Δ cells accumulate higher levels of zinc in the cytosol under zinc-limiting conditions.

(A, B, and C) cis4Δ cells expressing ZapCY1 were grown and subject to zinc shock as described in Fig 3B. Panel A shows a representative experiment and panel B shows the average values from 3 independent experiments with error bars representing standard deviations. Panel C shows a comparison of a 1 μM zinc shock in wild-type and cis4Δ cells expressing ZapCY1. (D) cis4Δ ZapCY1cells were grown as described in Fig 3B. Cells were transferred to temperature-controlled cuvettes and at the indicated times were exposed to +/- 50 μM NaPT or 0, 1, or 1000 μM Zn²⁺. Changes in the FRET ratio were determined as described in Fig 3B. Results show the average values from 3 independent experiments with error bars representing standard deviations. (E-G) The FRET ratio was measured in zrg17Δ cells expressing ZapCY1 as described in panel C. (H and I) cis4Δ and zrg17Δ cells expressing ZapCY2 were grown and subject to zinc shock as described in Fig 3B. Each panel shows the average values from 3 independent experiments with error bars representing standard deviations.