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. 2018 Mar 12;14(3):e1007263. doi: 10.1371/journal.pgen.1007263

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© 2018 Thapliyal et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) Expression of GFP under isoform-specific casy-1 promoters. casy-1a transcriptional reporter does not co-localize with mCherry marked cholinergic or GABAergic motor neurons. casy-1b and casy-1c expression reporters show expression in both cholinergic and GABAergic motor neurons. Anterior is to the left in all panels. Scale bar, 8μm. (B) Representative fluorescent images of Pcasy-1c::CASY-1C::mCherry translational reporter showing co-localization with the GABAergic nuIs376 [Punc-25::SNB-1::GFP] pre-synaptic markers suggesting the presence of CASY-1C in the GABAergic NMJ pre-synaptic termini. Scale bar, 10μm. (C) Representative fluorescent images of Pcasy-1c::CASY-1C::mCherry translational reporter showing co-localization with the cholinergic nuIs152 [Punc-2129::SNB-1::GFP] pre-synaptic markers suggesting the presence of CASY-1C in the cholinergic synapses. Scale bar, 10μm.