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. Author manuscript; available in PMC: 2018 Mar 22.
Published in final edited form as: Cell Chem Biol. 2017 Feb 2;24(2):231–242. doi: 10.1016/j.chembiol.2017.01.002

Figure 2.

Figure 2

Assessing the reactivity of a thioester reporter in complex proteomes. (a) Strategy for ex situ (lysate) and in situ (cellular) labeling using thioester 1. (b) Dose-dependent ex situ labeling of cancer cell lysates by thioester 1 (A549, 15 h, 0, 100, 200, 400, 800 μM). (c) Labeling by 1 is competed by acetyl-CoA (1 h pre-incubation with 0, 100, 200, 400, or 800 μM acetyl-CoA, then 400 μM 1 for 15 h). (d) Dose-dependent in situ labeling of cancer cell by thioester 1 (A549, 15 h, 0, 100, 200, 400, 800 μM).