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. Author manuscript; available in PMC: 2018 Jul 2.
Published in final edited form as: FEBS Lett. 2017 Jul 2;591(14):2091–2105. doi: 10.1002/1873-3468.12718

Fig. 2. Overexpressing microRNAs in DRG neurons in culture influences neurite length.

Fig. 2

DRG neurons were transfected with a control EGFP-anti-luciferase-siRNA expressing vector (A, B, C) or EGFP-microRNA expressing vectors (D-I) and let grow in vitro to analyze their effect on neurite growth. The neurons were immunostained for βIII tubulin (B, E, H) and automatically traced (C, F, I). The figure reports an example of one miRNA increasing the neurite length, miR-9 (D, E, F), and one decreasing length miR-466d (G, H, I). Scale bar is 200 μm.