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. Author manuscript; available in PMC: 2018 Jul 2.
Published in final edited form as: FEBS Lett. 2017 Jul 2;591(14):2091–2105. doi: 10.1002/1873-3468.12718

Fig. 5. qPCR confirms isoform differential expression.

Fig. 5

We used quantitative Real Time PCR to confirm differential expression for 8 selected isoforms. (A) Fold Changes of differential expression obtained using the Canx gene isoform for normalization strongly correlates (Pearson correlation R2 = 0.89, Kendall correlation tau coefficient = 0.71) with results reported by RNA-Seq analysis. (B) Similar results were obtained when using Psmb2 for normalization (Pearson correlation R2 = 0.9, Kendall tau coefficient = 0.71).