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. 2018 Mar 21;38(12):2923–2943. doi: 10.1523/JNEUROSCI.1532-17.2018

Figure 7.

Figure 7.

TSP-receptor α2δ-1 is synaptically expressed in the retina, and its expression is reduced in RCS rats. A, Representative images of the retina stained for α2δ-1 from LE (healthy) and RCS (degenerative) retinas at P14 and (B) P30. C, Quantitative staining intensity analysis demonstrated that α2δ-1 expression was reduced in RCS rat as early as P14. D, α2δ-1 was enriched in both the OPL and IPL, and the expression gap became more distinct at P30. Representative images of the (E) OPL and (F and G) IPL with the synapses labeled for Bassoon (F, green), VGluT1 (G, green), α2δ-1 (red), and NR1 (blue) from LE retina on P21 demonstrated postsynaptic expression of α2δ-1. Representative images of the (H) OPL and (J) IPL synapses labeled for Bassoon (green) and α2δ-1 (red) from LE (healthy) and RCS (degenerative) retinas on P21. Quantification of α2δ-1-containing synapses formed in the (I) OPL and (K) IPL reveals that the number of α2δ-1 synapse is already reduced in RCS rat by P21. Data were obtained from a minimum of 3 animals of either sex and expressed as mean ± SEM. ***p < 0.0001.