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. 2017 Dec 13;25(4):661–676. doi: 10.1038/s41418-017-0005-3

Fig. 7. γ-Secretase inhibitors suppress JNK3 expression and JNK function and protect RGC survival after ONA.

Fig. 7

a–d Representative western blots show JNK3 expression and overall phosphorylative activation of JNK in SH-SY5Y-APP cells treated with vehicle or γ-secretase inhibitors a L-685,458 (5 μM) or c BMS 299897 (1 μM). b, d The bar charts show quantitative analysis of relative JNK3 expression and overall phosphorylative activation of JNK normalized to the GAPDH (n = 3 for each treatment). e Representative western blots show phosphorylative activation of JNK3 in retinae from vehicle-treated or γ-secretase inhibitor BMS 299897 treated mice 2 weeks after ONA. f Representative fluorescence microscope images show immunostaining of mouse retina flat-mounts from vehicle or γ-secretase inhibitor BMS 299897 treated mice 2 weeks after ONA using anti-Tuj1 antibody. Scale bar, 50 μm. g The bar chart shows quantitative analysis of the surviving Tuj1+ RGC number in injured mouse retinae 14 days after ONA (n = 4 for each treatment). Student’s t-test: **p < 0.01. Error bars represent SEM