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. 2018 Jan 5;25(4):747–764. doi: 10.1038/s41418-017-0015-1

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© ADMC Associazione Differenziamento e Morte Cellulare 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

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Fig. 1 — a–f Immunocytochemistry revealed the expression of GPR125 (a), GFRA1 (b), UCHL1 and PLZF (c), and MAGEA4 (e) in human GPR125⁺ spermatogonia by MACS. Replacement of primary antibodies with isotype IgGs d, f were utilized as negative controls. Scale bars in a–f = 10 μm. The data shown in a–f were presented from three independent experiments. g RT-PCR showed the transcripts of GPR125, GFRA1, PLZF, UCHL1, RET, SYCP1, SYCP3, TNP1, ACR, PRM1, and PRM2 in the isolated human GPR125⁺ spermatogonia. ACTB was used as a loading control of total RNA. h Real -time PCR revealed the relative expression levels of PLZF, GPR125, GFRA1, UCHL1, RET, MAGEA4, SYCP1, SYCP3, TNP1, ACR, PRM1, and PRM2 to the housekeeping gene ACTB in the isolated human GPR125⁺ spermatogonia. The results shown in g and h were presented from three independent experiments