Table 6.
Genetically encoded fluorescent reporters of pH that have been successfully targeted to neuronal mitochondria
| Reporter name | Reporter type | Excitation, emission wavelength (nm) | pKa | References |
|---|---|---|---|---|
| MIMS-EYFP | Single FP (EYFP) | λex, ~513 | ~7.0 | [137, 138] |
| λem, ~530 | ||||
| mt-EYFP, mito-EYFP | Single FP (EYFP) | λex, ~513 | ~7.0 | [100, 137] |
| λem, ~530 | ||||
| mitoSypHer | Single FP (cpYFP), dual-excitation, ratiometric | λex, ~430, ~490 | ~8.7 | [141, 142] |
| λem, ~535 | ||||
| mtAlpHi | Single FP (EYFP) chimera | λex, ~500 | ~8.5 | [143] |
| λem, ~520 |
Publications refer to the work that originally generated the probe and studies where the probe was utilised in neuronal mitochondria. See text for more details