Table 7.
Fluorescent reporters of reactive oxygen species (ROS) that have been successfully targeted to neuronal mitochondria
| Reporter name | Reporter type | Peak excitation, emission wavelengths (nm) | References |
|---|---|---|---|
| mito-Het | Fluorescent molecule | λex, ~543 | [102, 153] |
| MitoSOX Red | λem, ~585 | ||
| MitoPY1 | Fluorescein/rhodamine fluorescent molecule | λex ~510 | [168, 195] |
| λem, ~528 | |||
| mito-roGFP | Single FP (dual-excitation, ratiometric) | λex, ~395, ~470 | |
| roGFPm | λem, ~510 | [169, 171, 172] | |
| Mito-Grx1-roGFP2 | Single FP (dual-excitation, ratiometric) | λex, ~395, ~488 | [142, 173] |
| λem, ~510 |
These reporters do not have absolute specificity for any single ROS type, and the accurate measurement of mitochondria-derived ROS is associated with various drawbacks, as outlined in the text. Publications refer to the work that originally generated the probe and/or studies where the probe was utilised in neuronal mitochondria