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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Steroids. 2017 Nov 10;133:15–20. doi: 10.1016/j.steroids.2017.10.018

Figure 1.

Figure 1

N27 cells express AR45 protein in membrane lipid rafts. N27 cells were treated with 100 nM testosterone for 24 hours to stabilized the androgen receptor. N27 cells were homogenized and separated into membrane, cytosol, and nuclear fractions. The membrane portion of the cells were further separated into 9 fractions using a sucrose gradient and ultracentrifugation in order to examine lipid rafts. Primary antibodies targeting AR45 (Santa Cruz sc-815/AR-C19 androgen receptor antibody) and lipid raft markers (Cell Signaling 3267 caveolin-1 antibody) were used. AR45 was only observed in caveolin positive lipid raft fractions. N=3 per treatment group.