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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: Exp Neurol. 2018 Feb 3;303:1–11. doi: 10.1016/j.expneurol.2018.01.019

Figure 5. OGD-induced depolarization increases with more astrocytes coupling into a syncytium.

Figure 5

(a) SR101 staining of a single freshly dissociated astrocyte, and three “miniature syncytia” with a varied number of astrocytes as indicated. AS: astrocyte. (b) OGD-induced VM depolarization increases with the number of coupled astrocytes. (c) Quantification of OGD-induced ΔVM in (b) (NS, P>0.05; *, P<0.05, ANOVA). (d) Representative astrocyte whole-cell currents recorded before and at the end of 30 min OGD under uncoupled and varied coupling syncytial sizes. (e) Normalized I-V plots show an OGD-induced positive shift in I-V plot (depolarization), but no suppression of passive conductance change in astrocyte in situ. (f) OGD induced neither a shift in I-V plot nor reduction in passive conductance in single astrocytes.