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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Metallomics. 2018 Feb 27;10(3):370–377. doi: 10.1039/c8mt00015h

Fig. 5.

Fig. 5

FRET images of ferroxidase, iron permease pairs. (a) Fluorescently-tagged Fet3 and Ftr1 were heterologously expressed in fet3Δftr1Δ S. cerevisiae. The FRET image shown reflects the increase in donor fluorescence upon photobleaching the acceptor. The FRET efficiency was 13.5% 12. (b) Fluorescently-tagged Fpn and Hp were heterologously expressed in HEK293T cells. The FRET image was generated as before. The efficiency was 9.8% which likely under-reports the biophysical energy transfer because it is uncorrected for donor fluor (Fpn-CFP) not in complex with Hp-YFP (Dlouhy and Kosman, unpublished).