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colocalization of megamitochondria with copper puncta. fluorescently labeled megamitochondria (a, cyan) overlap with copper puncta in nanosims images (b, magenta) of the same tissue. megamitochondria are indicated with arrows. asterisks denote colocalization of fluorescence and copper signals (c). inl: inner nuclear layer; opl: outer plexiform layer; onl: outer nuclear layer; rpe: retinal pigmented epithelium. scale bar: 5 μm.
