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. 2018 Mar 22;8:5075. doi: 10.1038/s41598-018-23419-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Real-Time PCR and NanoString analysis showing stimulatory response of Orientin on MEP transcriptional factors and key regulators of megakaryopoiesis. RNA isolated from mouse bone marrow mononuclear cells was used for Real Time quantitative RT-PCR analysis of (a) transcriptional factors of megakaryopoiesis at day 9 after irradiation and (b) associated genes at day 9 and 18 and (c) NanoString analysis of RNA from mouse BM mononuclear cells using genes from mouse inflammation panel at day 9. Histograms show fold change in ORI + IR vs IR. The megakaryocyte genes are in green and erythroid genes in red. Statistical significance was calculated using a Student t-test. For NanoString data, two-way ANOVA was performed for statistical significance between IR and ORI + IR group (n = 3 each).