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. 2018 Mar 22;8:5035. doi: 10.1038/s41598-018-23217-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of integrated platform and workflow. (a) Pre-enrichment platform is connected in-line with the BD Influx™ cell sorter. (b) Whole blood is labeled with antibodies against CTC markers as well as magnetic microparticles that bind unwanted blood cells. The sample then passes through a magnetic depletion step that removes >98% of unwanted blood cells followed by an in-line acoustic focusing and washing step, which removes debris and concentrates the sample prior to cell sorting. The sample can be interrogated based on cell markers and single cell or bulk populations of interest can be easily index-sorted for correlation of flow phenotype with molecular profile. WBC – White blood cell, RBC – Red blood cell, CTC – Circulating tumor cell, PZT – lead zirconate titanate.