Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 22;8:5035. doi: 10.1038/s41598-018-23217-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Whole transcriptome analysis of sorted single and pooled cells. (a) RNA sequencing was conducted on single cells (n = 11), 10-cell (n = 12) and 100-cell (N = 6) pools, and bulk RNA (n = 2) from PD798 cells pre-enriched and sorted from whole blood. Expression of selected hematopoietic lineage genes is extremely low or undetectable in all sorted cell populations, indicating high purity. Scale refers to log 2 TPM (transcripts per million). (b) Principal component analysis of gene expression of the 1,000 most variable genes in the different sorted cell populations or bulk RNA. (c) Correlation matrix comparing gene expression profile (13,817 genes) of single cells to the sum of all profiles (Synth 10) and the average of 10 cell pools (Ave 10). Scale refers to Pearson correlation coefficient. SC: Single cell.