Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 22;8:5035. doi: 10.1038/s41598-018-23217-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Integration of single cell sorting with BD Precise™ assay. (a) Heat map showing unsupervised hierarchical clustering of Molecular Index (MI) counts of 88 transcripts representing 78 genes in PD798, PD483, and WBCs. For visualization purposes, genes with total counts lower than 64 MI (1 SD below mean) were filtered out. Scale bar refers to log 2 MI counts. Expression of EPCAM and ECAD protein, as detected by flow cytometry, is denoted above heat map. (b) t-SNE analysis of gene expression of PD798 (triangle), PD483 (circle) and WBCs (inverted triangle) colored by log 10 mean fluorescence intensity (MFI) of CD45 (upper left), ECAD (upper right), EPCAM (lower left), and YFP (lower right). (c) t-SNE plots colored by expression of cell cycle genes Bub1, Ccnb1, Mcm6, Rrm2, Rrm2.1 (designates alternate Rrm2 transcript), and Plk1 (starting with upper left and moving clockwise). Scale bar refers to log 2 MI counts.