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. 2018 Mar 22;8:5047. doi: 10.1038/s41598-018-23454-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The expression of ErbB receptors and NRG1 isoforms is modulated in muscle after nerve injury. Three different types of nerve injury and injury/repair were performed on the median nerve (denervation, crush and end-to-end repair). At different time points, SDF muscle was collected and used for expression analysis. Graphs show the results of quantitative real-time PCR analysis. The analysis was performed on three animals per group; data are expressed as the mean ± SEM. Statistical analysis comparing groups at each single time point: one-way ANOVA plus Bonferroni’s post hoc test: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. E-E: end-to-end repair.