Figure 7.

Intravenous administration of MSC-spheroids is not associated to adverse reactions. (A) Body weight and (B) total fluid intake of animals that had consumed ethanol for 12 weeks and were intravenously injected with a single dose of 1 × 10⁶ MSC-spheroids or vehicle. Total fluid intake is expresses as ml of water + water in ethanol consumed/kg body weight/day. (C) Motor coordination evaluated forty days after MSC-spheroid or vehicle administration measuring the retention time using the rotarod test. (D) Exploratory behavior examined in a circular arena (open field) forty days after MSC-spheroid administration measuring locomotion, time spent without motion and time spent in the center of the arena. (E) Apoptotic rate in the hippocampus evaluated in brain sections by the TUNEL technique. Data are presented as percentage of TUNEL positive cells/DAPI/mm². The administration of MSC-spheroids to alcohol consuming animals did not modify the parameters assessed. Data are shown as mean ± SEM. N = 6 per experimental condition.