Fig. 6.

Nucleolar stress enhances PHA-4 binding and transactivates the expression of lipogenic genes. a Transcriptional expression of lipid metabolic genes using transcriptome profile analysis between WT and rrp-8(kun54) mutant worms. Abbreviations: ACDH, acyl-CoA dehydrogenase; ACS, acyl-CoA synthetase; CPT, carnitine palmitoyltransferase; ECH, enoyl-CoA hydratase; FA, fatty acids; HACD, hydroxyacyl-CoA dehydrogenase; PL, phospholipid; TAG, triacylglycerol. b Relative mRNA expression of dgat-2, fasn-1, and pod-2 in different worm strains or treatments. Data are presented as the means ± SD of four biological repeats. Significant difference between WT and a specific worm strain, ANOVA, ***P < 0.001, **P < 0.01, *P < 0.05. Significant difference between a specific worm strain with and without the pha-4(zu225) background, Student’s t-test, ^###P < 0.001, ^##P < 0.01, ^#P < 0.05. NS, no significant difference. c Potential PHA-4-binding sites in the promoter region of the lipogenic genes fasn-1, pod-2, and dgat-2. The green rectangles were reported in the modENCODE GFP ChIP-seq analysis and the blue dots were predicted based on the characterized PHA-4-binding elements. #1, #2, and #3 represent different primer pairs in the promoter region of different genes for ChIP-QPCR detection. d–f ChIP-QPCR analysis of PHA-4 bound to the promoters of the fasn-1, pod-2, and dgat-2 genes. d Pdgat-2 #2, e Pfasn-1 #2 and f Ppod-2 #2 indicate the #2 primer pair in the promoter region of respective genes used for ChIP-QPCR analysis. Data are presented as the means ± SD of three biological repeats