Skip to main content
. 2018 Mar 16;9:483. doi: 10.3389/fimmu.2018.00483

Figure 3.

Figure 3

β-catenin activity suppresses the IL-12/interferon gamma (IFN-γ) axis upon α-galactosylceramide (α-GalCer) challenge. (A) Percentages of CD3+NK1.1+ natural killer T cells in liver tissue after overnight challenge with ICG001, an inhibitor of β-catenin activity, or DMSO. After α-GalCer challenge, (B) serum concentrations of IFN-γ were determined by ELISA. The mRNA expression of Ifng (C) and Tbx21 (D) in the liver was determined by quantitative RT-PCR. (E) mRNA expression of Il12b in the liver and (F) protein expression of IL-12p40 in the serum. Dotted line represents average concentration of serum IL-12p40 for all mock-injected mice. Data are means ± SEM from four to nine mice per treatment for each time point cumulative from two to three independent experiments. Groups were compared by unpaired t test at each time point; *p < 0.05, **p < 0.01, N.D., not detectable.