Fig. 7. Taurine attenuates expression and activation of NOX2 induced by P + M.

aAfter 6 weeks of intoxication, the levels of NOX2 subunits p47^phox and gp91^phox were determined in the midbrain of P + M-treated mice with or without taurine by western blot using specific antibodies and the representative blots were shown. b The band density of blots was quantified. c After 6 weeks of intoxication, the levels of 4-HNE were determined in the midbrain of P + M-treated mice with or without taurine by western blot and the density of blots was quantified. d The membrane translocation of NOX2 cytosolic subunit, p47^phox was detected in microglial cells using western blot and the density of blots was quantified. Gp91phox and GAPDH were used as internal membrane and cytosolic control, respectively. e Microglial cells were treated with P + M with or without taurine pre-treatment. The production of superoxide was assessed by DHE and the density of red fluorescence of DHE oxidation was quantified. *p<0.05, **p < 0.01