Fig. 2. BPIFB1 regulated the CNE2 nasopharyngeal carcinoma cell radioresponse by interacting with VTN.

a BPIFB1 and VTN expression were confirmed by western blotting in CNE2 cells transfected or co-transfected with BPIFB1-Flag and VTN-His vectors, using anti-Flag and anti-His primary antibodies. b Clone formation assay showing the response of CNE2-NC (negative control), CNE2-BPIFB1, CNE2-VTN, and CNE2-BPIFB1/VTN cells to 0–8 Gy radiotherapy. c The numbers of surviving foci in the four groups are presented as bar graphs representing means ± SD. d Effects of BPIFB1 and VTN on survival of CNE2 cells after radiotherapy. Surviving fractions were calculated as described based on the data from experiments in c. e CCK8 assays were used to investigate the role of BPIFB1 and VTN in CNE2 cell proliferation after irradiation. *P < 0.05; **P < 0.01; ***P < 0.001; NS, no significance