Fig. 3. Overexpression of BPIFB1 inhibited VTN-induced anti-apoptotic effects in CNE2 nasopharyngeal carcinoma cells after irradiation.

a (Top) BPIFB1-overexpressing cells are sensitive to IR-induced cell death. The four groups of CNE2 cells were treated with or without 6 Gy of ionizing radiation and cells were stained with annexin V to measure the percentage of apoptotic cells. (Below) The percentage of apoptotic cells is presented as bar graphs representing means ± SD. ***P < 0.001; NS, no significance. b Expression of typical apoptosis markers, including cleaved caspase-9, cleaved caspase-3, cleaved caspase-7, and cleaved PARP, based on western blotting, comparing the four groups of CNE2 cells transfected or co-transfected with the BPIFB1-Flag and VTN-His vectors. GAPDH was used as an internal control. NC negative control. The numbers below blots represent grayscale values of each blot. The molecular weights of blots are indicated to their right