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. 2018 Mar 22;9(4):431. doi: 10.1038/s41419-018-0415-2

Fig. 2. Androgen ablation induces autophagy in prostate stromal fibroblasts.

Fig. 2

a Western blot data showing phospho-mTOR (p-mTOR), mTOR, AR, p62, and Beclin-1 expression levels and the LC3 conversion ratio (LC3-II/β-Actin) b in WPMY-AR cells. The cells were cultured with 0, 1, and 10 nM DHT, respectively, for 48 h, and treated with 50 nM RAPA or 50 μM CQ for 3 h before protein extraction. c, d WPMY-AR cells were infected with mRFP–GFP–LC3 adenovirus and cultured with 0, 1, and 10 nM DHT, respectively, for 48 h, and treated with 50 nM RAPA or 50 μM CQ for 3 h before 4% paraformaldehyde fixation and DAPI counterstaining. Scale bar, 5 μm. Bar graph showing LC3 puncta formation in different groups. e, f Transmission electron micrographs for detecting autophagic vacuoles in WPMY-AR cells cultured with 0, 1 and 10 nM DHT for 48 h, respectively, and treated with 50 nM RAPA for 3 h before 2% glutaraldehyde fixation. Enlarged images show double-membrane autophagic vacuoles. Scale bar, 1 μm. *P < 0.05, **P < 0.01