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. 2018 Mar 16;9:346. doi: 10.3389/fpls.2018.00346

FIGURE 3.

FIGURE 3

(A) A linear map of MuWRKY3 overexpressing vector with GUS and NptII marker genes for screening, 35S promoter and polyA terminator. Entire expression cassette is flanked by left and right borders in pCAMBIA2301. (B) Screening of transgenic and WT plants on half strength MS medium containing 200 mg/lit kanamycin and their acclimatization. (C) GUS histochemical staining of WT and transgenic seedlings at T3 generation. (D) PCR Amplification of MuWRKY3 gene GUS and NptII gene from genomic DNA of transgenic and WT plants: M, gene ruler mix; WT, wild-type; TR1 to TR10, transgenic lines.