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. 2018 Mar 22;8:5063. doi: 10.1038/s41598-018-23245-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fellow chart of the employed strategy in this study for extracting the Alternative Splicing events of transcript IDs through building a SpliceGraph. In the first phase, query transcript exons and all known exons of protein-coding transcripts of the related gene is downloaded using XML web service format of Ensembl. In the next phase, exons which do not follow the SpliceGraph construction rules is eliminated and consequently, SpliceGraph is built by remained exons coordinates, and in the last phase, query transcript exons are compared with SpliceGraph exons to extract alternative splicing types.