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. 2018 Mar 22;8:5052. doi: 10.1038/s41598-018-23194-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of VTN and P47 domain/SERA5. (a) (i) Representative immunofluorescence assay (IFA) images of merozoites and schizont. (ii) Upper panels, Representative IFA images of schizont. VTN was probed with an isotype control mAb instead of anti-VTN mAb. Lower panels, Representative IFA images of schizont cultured in AlbuMAX-I medium. Target proteins were probed with anti-VTN mAb (green), anti-SE36 rabbit serum (red), and DAPI nuclear stain (blue). Scale bar, 5 μm. (b) Representative IFA images of merozoite and schizont stages under deconvolution microscopy. Probes used were the same as in panel a. Upper panels show the localization of P47 domain/SERA5 during merozoite and schizont stages. Lower panels show the intensity of green and red signals at the section of the gray line in the upper panel. Scale bar, 1 μm. (c) Western blotting of NHS, red blood cell (RBC) lysate, and iRBC lysate. The blots were probed with anti-SE36 mouse serum (diluted 1:1000), anti-VTN pAb (diluted 1:2000), anti-albumin pAb (diluted 1:2000), and anti-band 3 mAb (diluted 1:2000). Band 3 is RBC-specific protein and used as loading control. (d) Representative IFA images of early trophozoite and trophozoite. Target proteins were probed with anti-VTN mAb (green), anti-SE36 rabbit serum (red), and DAPI nuclear stain (blue). Scale bar, 5 μm.