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. 2018 Mar 22;8:5052. doi: 10.1038/s41598-018-23194-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phagocytosis assay by THP-1 cells. (a) (i) Western blotting of bound protein(s) on latex beads after solubilization. Lane 1: SE36-beads, lane 2: SE36-beads with VTN, lane 3: latex-beads alone, lane 4: beads with VTN. SE36 and VTN bound to the beads were detected by anti-SE36 mouse serum (diluted 1:1000) and anti-VTN pAb (diluted 1:2000), respectively. Arrows indicate the target proteins. (ii) Representative flow cytometry (FCM) histogram of the antibody-independent engulfment of SE36-beads with or without VTN by THP-1 cells. (iii) Giemsa staining of engulfed SE36-beads (left panel) and non-treated beads (right panel) by THP-1 cells. Arrows indicate the engulfed SE36-beads. Scale bar, 10 μm. (b) FCM histogram of the engulfment by THP-1 cells of SE36-beads with or without VTN and/or purified IgG from NHS or HTS. (c) FCM histogram of the engulfment by THP-1 cells of SE36-beads treated with normal serum or VTN-depleted serum (VTN-depl). (d) FCM histogram of the engulfment by THP-1 cells of SE36-beads with or without VTN-depl, with or without purified VTN or with normal serum.