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. 2018 Mar 22;8:5056. doi: 10.1038/s41598-018-23354-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vitro properties of S. mutans MT8148 in the late logarithmic phase in the presence of various concentrations of S-PRG eluate. (A, B) Bacterial growth by adding 1 × 10⁹ CFU/ml of S. mutans with 18-h incubation, which was determined by OD₅₅₀ values in BHI broth (A) and recovered bacterial numbers on MSB plates (B). (C) Bacterial growth by adding 1 × 10⁹ CFU/ml of S. mutans at multiple time points, which was determined by OD₅₅₀ values in BHI broth. (D) Sucrose-dependent adhesion rates. (E) Quantitation of biofilm formation. Significant differences were determined using ANOVA with Bonferroni correction. *P < 0.05, **P < 0.01, and ***P < 0.001 versus no S-PRG eluate.