Table 5.
List of downregulated genes of MT8148 in the presence of S-PRG eluate, which were observed in either MT8148 or UA159 under three different conditions using DNA microarray analysis.
| Gene | Accession number | S-PRG 0% | S-PRG 6.3% | S-PRG12.5% | S-PRG 25.0% | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal | Signal | Log2* | Change# | Signal | Log2* | Change# | Signal | Log2* | Change# | ||
| bcc | 721410.1 | 3656.2 | 2409.2 | −0.6 | 0.659 | 2386.3 | −0.61 | 0.653 | 1414.7 | −3.81 | 0.071 |
| celR | 721935.1 | 34392.8 | 18718.7 | −0.88 | 0.545 | 7975.4 | −2.11 | 0.232 | 11089.6 | −1.63 | 0.323 |
| cilA | 721415.1 | 2407.2 | 1780.9 | −0.43 | 0.741 | 2047.7 | −0.23 | 0.851 | 1084.1 | −1.15 | 0.451 |
| cilB | 721414.1 | 2040.5 | 1377.5 | −0.57 | 0.675 | 1407.6 | −0.53 | 0.69 | 719.7 | −1.5 | 0.353 |
| cilG | 721413.1 | 1982.2 | 1594.2 | −0.31 | 0.804 | 1700.8 | −0.22 | 0.858 | 719.2 | −1.46 | 0.363 |
| citC | 721405.1 | 350.4 | 534.4 | 0.61 | 1.524 | 757.7 | 1.11 | 2.161 | 165.1 | −1.09 | 0.47 |
| citG | 721406.1 | 471.1 | 806.7 | 0.78 | 1.713 | 1094.5 | 1.22 | 2.324 | 143.2 | −1.72 | 0.303 |
| citG2 | 721416.1 | 3123.7 | 2413.7 | −0.37 | 0.772 | 2832.3 | −0.14 | 0.907 | 1131.6 | −1.47 | 0.362 |
| coaA | 721513.1 | 186.1 | 250 | 0.43 | 1.344 | 329.3 | 0.82 | 1.767 | 79.9 | −1.22 | 0.429 |
| comEA | 721053.1 | 264.2 | 352.6 | 0.42 | 1.335 | 521.8 | 0.98 | 1.975 | 32.2 | −3.04 | 0.122 |
| comYA | 722285.1 | 151.4 | 221.1 | 0.55 | 1.463 | 343.8 | 1.19 | 2.276 | 22.1 | −2.78 | 0.145 |
| comYB | 722284.1 | 253.4 | 374 | 0.56 | 1.479 | 515.5 | 1.03 | 2.042 | 46.4 | −2.44 | 0.184 |
| comYC | 722283.1 | 276.9 | 452.1 | 0.71 | 1.635 | 684 | 1.31 | 2.473 | 69 | −2 | 0.249 |
| comYD | 722282.1 | 147.2 | 263.5 | 0.85 | 1.801 | 402.7 | 1.45 | 2.724 | 43.7 | −1.76 | 0.295 |
| galR | 721293.1 | 1196.1 | 1062.5 | −0.17 | 0.888 | 1628.9 | 0.44 | 1.36 | 347.9 | −1.78 | 0.291 |
| grpE | 720560.1 | 24097.8 | 10856.3 | −1.15 | 0.451 | 10952.4 | −1.14 | 0.454 | 3039.2 | −2.99 | 0.126 |
| hsdM | 721299.1 | 103.1 | 17.6 | −2.55 | 0.171 | 14.2 | −2.86 | 0.138 | 40.4 | −1.35 | 0.392 |
| lacA | 721844.1 | 503.8 | 491.8 | −0.04 | 0.975 | 691.5 | 0.46 | 1.371 | 77.4 | −2.71 | 0.153 |
| lacB | 721843.1 | 1420.2 | 1345.4 | −0.08 | 0.947 | 1602.7 | 0.17 | 1.129 | 174.2 | −3.03 | 0.123 |
| lacC | 721842.1 | 693.3 | 681.8 | −0.02 | 0.984 | 733.5 | 0.08 | 1.06 | 104.1 | −2.73 | 0.15 |
| lacD | 721841.1 | 791.7 | 947.8 | 0.24 | 1.184 | 786.9 | −0.01 | 0.994 | 88.4 | −3.16 | 0.112 |
| lacD2 | 720591.1 | 3352.2 | 1741.7 | −0.94 | 0.52 | 1563.4 | −1.1 | 0.466 | 103.9 | −5.01 | 0.031 |
| lacE | 721839.1 | 1851.5 | 2078.7 | 0.17 | 1.124 | 2250.3 | 0.28 | 1.216 | 210.6 | −3.14 | 0.114 |
| lacF | 721840.1 | 1250.6 | 1567.3 | 0.33 | 1.253 | 1506.8 | 0.27 | 1.205 | 127.4 | −3.3 | 0.102 |
| lacG | 721838.1 | 7506.5 | 7801.4 | 0.06 | 1.039 | 5603.4 | −0.42 | 0.747 | 783.6 | −3.26 | 0.104 |
| lacX | 721837.1 | 2089.4 | 2392.3 | 0.19 | 1.143 | 3630.3 | 0.8 | 1.737 | 211.3 | −3.31 | 0.101 |
| lytR | 721011.1 | 2102.5 | 1188.7 | −0.82 | 0.565 | 1104.9 | −0.93 | 0.526 | 507.7 | −2.05 | 0.241 |
| oadB | 721411.1 | 4735.5 | 3705.5 | −0.36 | 0.782 | 3979.2 | −0.25 | 0.84 | 2741.2 | −0.79 | 0.578 |
| pflC | 720930.1 | 968.8 | 781.5 | −0.31 | 0.806 | 904.2 | −0.1 | 0.933 | 645 | −0.59 | 0.664 |
| ptcA | 721934.1 | 21838.2 | 12188.5 | −0.84 | 0.558 | 4817.3 | −2.18 | 0.221 | 6571.4 | −1.73 | 0.301 |
| ptcB | 721936.1 | 1549.5 | 1220.1 | −0.35 | 0.786 | 1351.8 | −0.2 | 0.872 | 1410.3 | −0.14 | 0.91 |
| ptcC | 721932.1 | 8470.6 | 4527.1 | −0.9 | 0.534 | 2615.5 | −1.7 | 0.309 | 2737.8 | −1.63 | 0.323 |
| pycB | 721417.1 | 3315.9 | 2227.8 | −0.57 | 0.672 | 2493.8 | −0.41 | 0.752 | 2372.2 | −0.48 | 0.715 |
| rgpG | 720710.1 | 107407.9 | 3328.3 | −5.01 | 0.031 | 4296.3 | −4.64 | 0.04 | 4296.3 | −4.64 | 0.04 |
| scnE | 722129.1 | 47 | 23.3 | −1 | 0.5 | 14.1 | −1.74 | 0.3 | 4.2 | −3.47 | 0.09 |
| spaP | 721042.1 | 7581.6 | 3685 | −1.04 | 0.486 | 1858 | −2.03 | 0.245 | 1936 | −1.97 | 0.255 |
| ssb2 | 722266.1 | 673.6 | 980.5 | 0.54 | 1.459 | 1165.4 | 0.79 | 1.733 | 529 | −0.35 | 0.787 |
| trk | 721902.1 | 29059.7 | 13948.7 | −1.06 | 0.48 | 6713.2 | −2.11 | 0.231 | 5386.5 | −2.43 | 0.185 |
| trkB | 721901.1 | 21115.7 | 10376.4 | −1.03 | 0.491 | 4014.8 | −2.4 | 0.19 | 3970.1 | −2.41 | 0.188 |
| wapA | 721382.1 | 3431.3 | 1715.5 | −1 | 0.5 | 1588.4 | −1.11 | 0.463 | 1652.3 | −1.05 | 0.482 |
*Log2 means Log2 ratio. #Change means Fold change. The Log2 ratio and Fold change were calculated by signal with each concentration of S-PRG eluate/signal without S-PRG eluate.