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Fha detection by Western blotting. Fha production determined by ECL assay was found to be deficient in J014, J199, J042, J043, and B199 compared to Fha-producing isolates J010 and J365. Each well was loaded with 10 μg of total protein. The anti-Fha antibody recognized multiple polypeptides in J010, consistent with the frequent observation of various FhaB degradation products in B. pertussis cell extracts (28).
