FIG 3.

Observed mutations in fhaB. Mutations within domains of the FhaB preprotein: signal peptide (SP), two-partner secretion (TPS), mature C-terminal (MCD), N-terminal prodomain (PNT), proline-rich region (PRR), and extreme C-terminal (ECT) domains. Genomes of isolates J199, B199, and B3585 exhibited mutations that altered mature Fha, while J043 and J365 included IS481 insertion within the C-terminal prodomain. Predicted IS481 insertion target sites at positions 2785, 3124, and 9865 (RD16_09375 in E476) are indicated with red arrows.