FIG 7.

Expression of CpALS7 leads to increased adhesion. (A) CpALS7 was cloned into the S. cerevisiae expression vector pYES2 under the control of the galactose-inducible promoter. Yeast cells containing this plasmid or the vector only were grown with either glucose (Glu) (repressive) or galactose (Gal) (inductive) as the sole carbon source, and adhesion was measured in the microfluidics device with a shear force of 1 or 5 dynes/cm². Data are from duplicate samples at each shear force with 5 biological replicates. Comparisons between cells grown in Glu and those grown in Gal were made by using a Mann-Whitney rank sum test. *, P < 0.001 for Gal versus Glu at each shear force. (B) A C. parapsilosis strain harboring a homozygous deletion of CpALS7 (CpALS7^−/−) and the wild-type parent strain from which it was derived (19) were induced by growth in M199 for 3 h at 37°C and tested in adhesion assays as described above. The adhesion indices of induced (I) and control (C) yeast cells were calculated from duplicate channels with a minimum of 3 biological replicates. Statistical analysis was performed by using Kruskal-Wallis analysis of variance on ranks, and between-group comparisons were made by using Dunn's method. *, P < 0.001.