TABLE 2.
C. parapsilosis ALS and housekeeping gene homologues and primers
| Gene | Abbreviation or alternate gene name | qPCR primer | Primer sequence |
|---|---|---|---|
| CPAR2_500660 | 500660 | 500660_F | ATGGCTTGGATCAACCACTT |
| 500660_R | CAGAACTTACGGCAGGGGTA | ||
| CPAR2_404770 | 404770 | 404770_F | AACTGGCCATGAAAACAAGG |
| 404770_R | CCATAGCAGGTTTGCACAGA | ||
| CPAR2_404790 | 404790 | 404790_F | GGCGTCAAACACAGAATCAA |
| 404790_R | GCTGCTGTTGCTGTGACATT | ||
| CPAR2_404800 | CpALS7 | 404800_F | ACCACCACCGAGGTTACAAA |
| 404800_R | TGGATCTCCGGTTTTGAGTC | ||
| CPAR2_404780a | 404780 | 404780_F | CGCAAGTACTCCTTCCAACC |
| 404780_R | CCATTACCTTCGTTTCCATTG | ||
| CPAR2_201570 | CpACT1 | CpACT1_F | CGAACGTGGTTACGGTTTCT |
| CpACT1_R | TGACCATCTGGCAATTCGTA | ||
| CPAR2_502950 | CpPMA1 | CpPMA1_F | CGCCAACGAAGTTGTTCCTG |
| CpPMA1_R | AGATTCACCAGTGATGGCGG | ||
| CPAR2_806050 | CpRIP1 | CpRIP1_F | CCCAATTGGTGAAGCAGGTG |
| CpRIP1_R | CCAAGTTCAAAGGGGCTGGA | ||
| CPAR2_807570 | CpRPP2B | CpRPP2B_F | CTCCCCATCTGCTTCAGACA |
| CpRPP2B_R | TGAGGCCAATTTGGTGTTACCT | ||
| CPAR2_204740 | CpLSC2 | CpLSC2_F | TTGGACTGTGGTGGTACTGC |
| CpLSC2_R | TTGCGGCAATCAAACCCTTG | ||
| CPAR2_104580 | CpIMH3 | CpIMH3_F | TCACTGTTGGGGACGTCAAG |
| CpIMH3_R | GGATCGTCGTTGTTTTCGTGA | ||
| CPAR2_401060 | CpCPA1 | CpCPA1_F | CCCGAGAGCATGACTGATCC |
| CpCPA1_R | AGCAACATCACCAACGACGA | ||
| CPAR2_808670 | CpTDH3 | CpTDH3_F | ACCACCAACTGTTTGGCTCC |
| CpTDH3_R | ACCACCTCTCCAGTCCTTGT |
a
The primer set for CPAR2_404780 was noted to also recognize CPAR2_500660 during the review process. Analysis of qPCR data from samples containing these primer sets indicated that the expression level of CPAR2_500660 was extremely low and contributed ∼1% to the total mRNA levels at most. This observation is very consistent with previously reported data (19). Since the contribution of CPAR2_500660 to the calculations of CPAR2_404780 levels was negligible, the cross-hybridization of this primer set does not affect the data presented in a meaningful way.