Anti-VAR2CSA antibody levels are independent of parity. (A) Anti-VAR2CSA antibody levels were measured by ELISA at inclusion and at delivery in sera from Colombian primigravid (PG) and multigravid (MG) women, and levels were compared to those of a Colombian malaria-unexposed control group. (B) Matched antibody levels measured at enrollment and at delivery in individual subjects. Horizontal lines indicate the means for each group, error bars indicate SDs, and dotted horizontal lines mark the cutoff for seropositivity (AU, 19.7). ns, not significant.