FIG 2.

Antigen-specific CD8⁺ T cells initiate recruitment of activated CD8⁺ T cells. (A and B) Activated OT-I and 2C cells were incubated with (+) or without (−) PTX and transferred to C57BL/6 mice. The mice were uninfected (A) or infected (B) with PbA-gfpOVA sporozoites, and cell numbers in the liver were determined by flow cytometry. Each dot represents data for one mouse. (C) After infection with PbA-gfpOVA, numbers of OT-I and 2C cells in individual clusters were determined by using two-photon microscopy. Each dot represents data for one cluster. The values for OT-I and 2C cells in the same mouse (A and B) or in the same cluster (C) are connected by a line. Data are representative of data from three experiments with similar results. *, P < 0.05; ns, not significant (by two-way ANOVA followed by Sidak's multiple-comparison tests).