TABLE 1.
Gene target | Primer orientation | Sequence | Amplicon size (bp) | Reference |
---|---|---|---|---|
Human IDO1 | Forward | 5′-AGAAGTGGGCTTTGCTCTGC-3′ | 230 | 59 |
Reverse | 5′-TGGCAAGACCTTACGGACATCTC-3′ | |||
Human β-actin | Forward | 5′-CATGTACGTTGCTATCCAGGC-3′ | 250 | 60 |
Reverse | 5′-CTCCTTAATGTCACGCACGAT-3′ | |||
trpR | Forward | 5′-GATGGCTGGAATGCGTTTGT-3′ | 176 | This study |
Reverse | 5′-CTCACACCATATGCCTCAG-3′ | |||
trpB | Forward | 5′-AGACAGGAGCAGGACAAC-3′ | 198 | This study |
Reverse | 5′-GGAGAGCCTCATTTACGG-3′ | |||
16S rRNA | Forward | 5′-AGTCGAACGGAATAATGGCT-3′ | 204 | 62 |
Reverse | 5′-CCAACAAGCTGATATCCCAC-3′ |