FIG 5.

Percentages of nuclear DNA in the midguts of “Ca. Liberibacter asiaticus” exposed and nonexposed adult and 5th-instar nymph D. citri insects showing a normal (black) or fragmented (white) phenotype. These samples are referenced as “Ca. Liberibacter asiaticus” exposed rather than “infected” because they were stained with DAPI to observe nuclear DNA phenotypes and not the “Ca. Liberibacter asiaticus” probe. Among adults, the percentages fragmented were significantly different between nonexposed and exposed insects (W = 1,558; P ≪ 0.05), while the difference in nymphs was not significant (W = 670; P = 0.21). Among nonexposed samples, adults and nymphs had significantly different numbers of fragmented DAPI-stained nuclei (W = 2,195.5; P = 0.0001). Among exposed samples, adults and nymphs also had significantly different numbers of fragmented DAPI-stained nuclei (W = 3,024; P ≪ 0.05). The P values were generated using the Mann-Whitney U test in R.