FIG 6.

Percentages of nuclear DNA in the midgut of “Ca. Liberibacter asiaticus”-exposed and nonexposed adult and 5th-instar nymph D. citri insects showing the three fragmentation classes. These samples are referenced as “Ca. Liberibacter asiaticus” exposed rather than infected because they were stained only for DAPI to observe nuclear DNA phenotypes, not for the “Ca. Liberibacter asiaticus” fluorescent probe, as described in Materials and Methods. Among adults, nonexposed and exposed insects were significantly different for classes 2 and 3 (W = 1,180.5, P ≪ 0.05 and W = 1,034.5, P ≪ 0.05, respectively) but not different for class 1 (W = 586.5; P = 0.69). Among nymphs, nonexposed and exposed insects were not significantly different for classes 2 and 3 (W = 288, P = 0.63 and W = 255.5, P = 0.65, respectively), while class 1 was significantly different (W = 384; P = 0.009). P values were generated using the Mann-Whitney U test in R.