FIG 5.

Immunofluorescence colocalization of T. gondii GRA5 and GIMAPs in rat primary peritoneal cells infected with the T. gondii type I RH strain. Primary peritoneal cells freshly isolated from Lewis rat (A) and Brown Norway rat (B) were cultured with T. gondii tachyzoites for 12 h, followed by immunofluorescence analyses using combinations of anti-GIMAP antibodies (red stain, Alexa Fluor 594), anti-GRA5 antibody (green stain, Alexa Fluor 488), and a nucleus stain (DAPI). Images of the cells were also captured by differential interference contrast (DIC). In Lewis rat cells, GIMAP 4, 5, and 6 densely colocalized with GRA5 (Merge panels in panel A). In Brown Norway rat cells, a very low fluorescence signal of GIMAP 4 that partially overlapped the GRA5 signals was detectable, while GIMAP 5 and GIMAP 6 were undetectable (Merge panels in panel B).