FIG 6.

Immunofluorescence colocalization of T. gondii SAG1 and lysosome marker protein LAMP1 in NR8383 cells infected with the T. gondii type I RH strain. NR8383 cells engineered for inducible overexpression of GIMAP 4, 5_201, and 6, as well as NR8383 cells with the empty pLVX-TetOne-Puro expression vector, were induced with doxycycline for 24 h and thereafter cultured with T. gondii tachyzoites for 4 h, followed by immunofluorescence analyses using combinations of anti-LAMP1 antibody (red stain), anti-SAG1 antibody (green stain), and the DAPI nuclear stain (blue). In GIMAP 4-, 5_201-, and 6-expressing cells, LAMP1 colocalized with SAG1 (Merge panels), while in cells expressing the empty vector, LAMP1 was sparsely distributed in the host cell cytosol and did not appear to overlap SAG1 (Merge panels).