FIG 5.

Ability of rat antisera to the ICAM-1-binding motif in DBLβ_motif domains to inhibit binding of recombinant DBLβ domains to ICAM-1. (A) Antisera from rats immunized with M6pep or with both M6pep and M9pep tested against recombinant DBLβ_motif domains (M2 to M7, M9, and M11 to M13) and DBLβ_nonmotif domains (N27 and N33). Shading, DBLβ domain numbers, and antiserum specificities are as described in the legend to Fig. 3B. (B to D) Inhibition by the same antisera of ICAM-1-specific adhesion of PFD1235w-positive IEs (B), HB3VAR03-positive IEs (C), and IT4VAR13-positive IEs (D) under physiologic shear stress. The statistical significance of the reductions is indicated as described in the legend to Fig. 4. Three independent experiments were done (with three technical replicates in each). Fewer than 0.25 IEs/mm² bound to uncoated channels were observed. (E) Immunofluorescence of representative IEs with surface expression of PFD1235w (top row), HB3VAR03 (center row), and IT4VAR13 (bottom row) and labeled by sera from rats immunized with M6pep only (left column) or with both M6pep and M9pep (center column), or by a rat antiserum to N27 (right column). See Table S2 in the supplemental material for raw data.