FIG 2.

Clodronate treatment does not result in a bactericidal alveolar environment. (A) F. tularensis SchuS4 bacteria were incubated at 37°C for 1 h at a density of 273 CFU/ml (60 CFU in 220 μl) in MHB medium alone or in MHB medium containing 20% clodronate-containing liposomes or PBS-containing liposomes. The bacterial burden per milliliter was assessed by plating following incubation. (B) BALB/c mice were treated i.n. with liposomal clodronate or PBS or were left untreated 1 day prior to collection of BALF. F. tularensis SchuS4 bacteria were incubated at 37°C for 1 h in sterile-filtered BALF at a density of 300 CFU/ml (60 CFU in 200 μl). The bacterial burden per milliliter was assessed by plating following incubation. NS, not significant by one-way ANOVA.