TABLE 2.
Quantitation of prostaglandin species in lysates of trypomastigotes of T. cruzi by LC–MS-MS
| Prostaglandin lipid speciesa | Mass (m/z) of diagnostic ions at quadrupole position (Q): |
Amt in trypomastigotes of T. cruzi strainb: |
||
|---|---|---|---|---|
| Q1 | Q3 | Tulahuen | Brazil | |
| PGD2 | 351 | 189 | 1.4–3.9 | 2.7–23 |
| PGE2 | 351 | 189 | 2.0–21.4 | 6.0–48.6 |
| PGF2α | 353 | 193 | 2.6–38.9 | 2.7–2.6 |
a
PG, prostaglandin.
b
Expression of lipid species quantified from peak height and expressed as picograms per milligram of protein. The data represent the range of samples documented from 3 independent lysates for each strain.